顺铂与唑来膦酸序贯应用抑制肺癌细胞增殖的研究

目的: 探讨顺铂与唑来膦酸序贯联合应用对人肺巨细胞癌细胞株PLA-801D的生长抑制作用。方法: MTT法测定顺铂与唑来膦酸不同序贯方式处理后细胞生长的抑制率,并通过流式细胞术分析不同处理组细胞周期的变化。结果: 顺铂和唑来膦酸均能够抑制肿瘤细胞的生长,并在一定给药浓度范围内具有剂量依赖效应。两药联合应用对细胞生长抑制具有协同效应,其中以顺铂给药24 h后序以唑来膦酸处理对细胞生长的抑制效果最佳。顺铂与唑来膦酸序贯方式的改变,引起G0/G1期细胞比例下降,S期细胞比例显著提高。结论: 先顺铂后唑来膦酸的联合给药方式对肺癌细胞的生长抑制作用最强,不同序贯应用方式导致细胞生长抑制率的差异与细胞周期的变化具有一定的相关性。

Inhibitory effect of cisplatin combined with zoledronic acid on the cell proliferation of lung cancer

AIM: To investigate the inhibitory effect of cisplatin combined with zoledronic acid on lung cancer cell PLA-801D proliferation. METHODS: Cell growth inhibitory rate of cisplatin combined with zoledronic acid was determined with MTT assay. The cell cycle was detected by flow cytometry. RESULTS: Cell proliferation was inhibited under the treatment of cisplatin, zoledronic acid alone. The inhibitory rate showed a dose dependent effect within a certain drug concentration range. Compared with single-drug treatment, the combined treatment of cisplatin and zoledronic acid showed a synergistic effect on cell growth inhibition. The highest inhibitory rate was found in sequential cisplatin+zoledronic acid group (cells were first incubated with cisplatin followed by zoledronic acid treatment 24 h later). In addition, compared with the control group, the S phase was longer after combined treatment of cisplatin and zoledronic acid. CONCLUSION: Sequential treatment of cisplatin and zoledronic acid show the strongest inhibitory effect on the cell proliferation of lung cancer. The ranged inhibitory effect may be related with the change of cell cycle.