桑叶中1-脱氧野尻霉素对糖尿病肾病大鼠的治疗作用

目的:通过观察桑叶中1-脱氧野尻霉素（DNJ）对糖尿病肾病大鼠的血糖和肾损伤的影响，探讨桑叶生物碱在延缓糖尿病肾病发病进程中的疗效。方法:以高糖高脂饲料喂养SD大鼠4周后腹腔注射链脲佐菌素(STZ，35 mg/kg) 建立糖尿病肾病模型鼠，将建模成功的大鼠随机分成3组，即模型对照组、低剂量（10 mg/kg）、高剂量（25 mg/kg）的DNJ组，另设正常对照组，连续灌胃给药6周，分别在第0、2、4、6周末测量各组大鼠的空腹血糖（FBG），并在药物干预6周结束时进行口服糖耐量（OGTT）实验，测量大鼠体质量、肾脏重量，同时检测血尿素氮（BUN）和血肌酐（Scr）的水平，并收集尿液测定尿白蛋白/肌酐的比值。结合苏木精-伊红（HE）染色在电镜下观察大鼠肾组织病理学变化。结果:与模型对照组相比，糖尿病模型鼠在灌胃给予低剂量（10 mg/kg）、高剂量（25 mg/kg）的DNJ后，给药后第4，6周时血糖水平下降，灌胃6周后糖耐量也有显著性改善。6周末时，低、高剂量DNJ组大鼠的肾肥大指数均低于模型组，其尿白蛋白/肌酐比值也显著降低。低剂量的DNJ降低糖尿病肾病大鼠血中Scr水平，而高剂量的DNJ均能降低糖尿病肾病大鼠血中Scr和BUN水平。通过观察肾组织病理切片，低、高剂量的DNJ均能减轻糖尿病肾病模型大鼠的肾脏损伤。结论:桑叶中DNJ具有降血糖和减轻肾损伤的作用，在延缓糖尿病肾病发病进程上具有重要意义。

Therapeutic effects of 1-Deoxynojirimycin in mulberry leaves on rats with diabetic nephropathy

AIM: To explore the effect of 1-deoxynojirimycin (DNJ) from mulberry leaves on blood glucose and kidney injury on rats with diabetic nephropathy by investigating the effect DNJ on delay the pathogenesis of diabetic nephropathy. METHODS: Diabetic nephropathy model were established by intraperitoneal injection of streptozotocin (STZ, 35 mg/kg) in male SD rats, then the animals were randomly divided into 3 groups. Rats in low and high dose DNJ groups were given 10, 25 mg/kg DNJ for 6 weeks, and no drug was given to rats in model group. SD male rats were included in experiment as normal control group. Fasting blood glucose (FBG) were detected at the 0, 4th, 6th, 12th week, and an oral glucose tolerance (OGTT) was performed at the end of 6th week after drug intervention. The body mass, urine albumin/creatinine ratio, kidney mass, serum urea nitrogen (BUN) and serum creatinine (Scr) were detected. Histopathological changes of kidney were observed by hematoxylin-eosin (HE) staining. RESULTS: Compared with model group, intragastric administration of low dose (10 mg/kg) or high dose (25 mg/kg) of DNJ reduced blood glucose at 4th and 6th week in diabetic rats, glucose tolerance was also significantly improved at 6th week. After intragastric administration of low dose (10 mg/kg) or high dose (25 mg /kg) of DNJ once daily for 6 weeks, both the renal hypertrophy index and urine albumin/creatinine ratio of rats with diabetic nephropathy were lower than that of model group. Low dose of DNJ reduced the level of Scr,while high dose of DNJ could decrease both the levels of Scr and BUN in diabetic nephropathy rats. By observing the pathological sections of renal tissue, the renal injury of rats with diabetic nephropathy were significantly attenuated with low or high dose of DNJ treatment. CONCLUSION: The DNJ extracted from mulberry leaves reduce blood glucose and attenuate renal damage in rats with diabetic nephropathy, and may delay the pathogenesis of diabetic nephropathy.