电离辐射对人肝细胞MXR7基因表达的影响及放射性工作人员外周血MXR7的表达分析

为探讨电离辐射对人正常肝细胞及外周血MXR7基因表达的影响,利用⁶⁰Co γ射线对培养的人肝细胞HL-7702进行照射,同时,抽取某工厂放射性工作人员外周血,通过实时荧光定量PCR对照射后的各剂量组肝细胞及放射性工作人员外周血MXR7基因的表达情况进行检测分析。结果表明,电离辐射可诱导人正常肝细胞MXR7基因的差异表达,且基因表达与照射剂量存在一定的剂量相关性;放射性工作人员外周血MXR7基因表达与非放射性工作人员相比显著升高。

Detection and analysis of MXR7 gene expression in human liver cells induced by radiation and peripheral blood of radiation workers

In order to explore the changes of MXR7 genes expression in human liver cells HL-7702 and peripheral blood induced by radiation,the expression changes of MXR7 gene in human liver cells irradiated by ⁶⁰Co γ-ray were detected by real-time fluorescent quantitative PCR. Furthermore, MXR7 gene expression levels were detected in peripheral blood from radiation workers. Results showed that ionizing radiation can induce the change of MXR7 genes expression in human liver cells, and it is dose-related. The expression of MXR7 gene in peripheral blood from radiation workers was up-regulated to compare with the expression of non-radiation workers.