四君子汤对SGC-7901侧群细胞裸鼠的抑瘤作用及其机制

目的:探讨四君子汤对胃癌细胞株SGC-7901侧群（SP）细胞、非侧群（NSP）细胞裸鼠成瘤能力的影响。方法:常规培养人胃癌细胞株SGC-7901后进行流式细胞仪分选出SP及NSP细胞，SP细胞比例约为1.9%，建立裸鼠成瘤模型，药物干预组给予四君子汤灌胃连续干预。30 d后处理所有小鼠，绘制肿瘤生长曲线，测定肿瘤生长抑制率，TUNEL法检测凋亡细胞。 结果:SP全方组、NSP全方组及其各自模型组平均肿瘤体积分别为(1 348.40±103.98) mm3、(628.99±98.87) mm3；(3 307.76±491.44) mm3、(2 202.87±67.80) mm3（P＜0.05），SP及NSP全方组抑瘤率为54.69%、60.19%，较模型组有统计学差异（P＜0.05）；侧群细胞与非侧群组之间成瘤存在统计学差异（P＜0.05）。TUNEL结果显示药物组出现较多凋亡细胞，SP群全方组、NSP全方组的凋亡率分别为20.21%、16.53%，明显高于模型组（P＜0.05）。 结论:四君子汤可抑制裸鼠人胃癌SGC-7901 SP细胞瘤体生长，其抑瘤机制可能与肿瘤细胞凋亡有关。

Antitumoral effects of Sijunzi decoction on side population of SGC-7901 in nude mice and mechanism

AIM: To investigate the antitumor effect of Sijunzi decoction on side population (SP) cells and non-side population (NSP) cells of gastric cancer cell line SGC 7901 in nude mice. METHODS: Human gastric cancer cell line SGC-7901 SP cells were conventionally cultured, then, SP and NSP cells were sorted by flow cytometry. Proportion of SP cells was about 1.9%. The model of tumor-bearing mice was established and drug intervention group mice were given a gavage of Sijunzi decoction. All mice were killed after 30 days and the tumor growth curve was drawn and the tumor growth inhibition rate was measured. Tumor growth inhibition rate (IR) was calculated and apoptosis was detected by TUNEL method. RESULTS:The mean tumor volumes of SP-Drug group, NSP-Drug group were (1 348.40±103.98) mm³, (628.99±98.87) mm³, and their model group were (3 307.76±491.44) mm³, (2 202.87±67.80) mm³ (P＜0.05), respectively. Tumor inhibition rate of SP-drug group and NSP-drug group were 54.69% and 60.19%, which were significantly different from the control groups (P＜0.05). There was also a difference between SP group and the NSP group (P＜0.05). TUNEL results showed that there were more apoptotic cells in the drug groups. The apoptosis rate of SP group and NSP group were 20.21% and 16.53%, which were obviously higher than control groups (P＜0.05). CONCLUSION: Sijunzi decoction can inhibit the growth of human gastric cancer SGC-7901 SP cells in nude mice in vivo, which was probably related to induction of apoptosis in tumor cells.