Borago officinalis oil: Fatty acid fractionation by immobilized Candida rugosa lipase |
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| Authors: | Murielle Schmitt-Rozieres Guillaume Vanot Valérie Deyris Louis-Claude Comeau |
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| Affiliation: | 1. Laboratoire de Chimie Biologique Appliquée, Faculté des Sciences de Saint-Jér?me, Case 431, Avenue Escadrille Normandie-Niemen, 13397, Marseille Cedex 20, France
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| Abstract: | γ-Linolenic acid (Z,Z,Z-6,9,12-octadecatrienoic acid), a very important polyunsaturated fatty acid is found in the free fatty acid fraction prepared
by the hydrolysis of borage oil. Our aim was to enrich this fraction in γ-linolenic acid using selective esterification. Candida rugosa lipase was used as catalyst after immobilization on the following ion-exchange resins: Amberlite IRC50, IRA35, IRA93, and
Duolite A7, A368, A568. In every case, immobilization modified the lipae’s specificity: palmitic, stearic, oleic, and linoleic
acids were preferentially esterified compared to γ-linolenic acid, thus allowing a γ-linolenic acid enrichment of 3.0. |
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| Keywords: | Borago officinalis Candida rugosa enzyme immobilization fatty acid enrichment γ -linolenic acid lipase |
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