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| GC-MS法测定大花红景天中大花红天素含量 | |
| 引用本文： | 吴少雄,张闯,王保兴,郭祀远. GC-MS法测定大花红景天中大花红天素含量[J]. 食品与发酵工业, 2008, 34(1): 116-118 |
| 作者姓名： | 吴少雄 张闯 王保兴 郭祀远 |
| 作者单位： | 1. 昆明医学院营养与食品研究所,云南昆明,650031 2. 云南中医学院民族医药研发中心,云南昆明,650200 3. 云南瑞升科技有限公司,云南昆明,650223 4. 华南理工大学轻工与食品学院,广东广州,510640 |
| 摘 要： | 利用气相色谱-质谱(GC-MS)法测定大花红景天中的大花红景天素含量。样品中的大花红天素经水提取,大孔树脂柱净化、分离。根据MS的定性结果,强峰m/z 69为大花红天素的特征离子峰,利用选择性离子扫描方式(SIM)进行定量分析。实验结果表明:方法回收率在93.70%～98.58%,变异系数为1.23%～2.94%,线性范围在0.10～0.50 mg/ml,检出限(3S/N)为0.01 mg/mL,该分析方法具有准确度高、选择性好、线性范围宽、干扰少等特点,适合大花红景天中大花红天素的定量测定。 |
| 关 键 词： | 大花红天素 大花红景天 测定 气相色谱-质谱法 |
| 修稿时间： | 2007-10-10 |
Determination of Crenulatin in Rhodiola crenulata H.by Chromatography-Mass Spectrometry |
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| Wu Shaoxiong,Zhang Chuang,Wang Baoxing,Guo Siyuan. Determination of Crenulatin in Rhodiola crenulata H.by Chromatography-Mass Spectrometry[J]. Food and Fermentation Industries, 2008, 34(1): 116-118 | |
| Authors: | Wu Shaoxiong Zhang Chuang Wang Baoxing Guo Siyuan |
| Abstract: | An determination method of Crenulatin in Rhodiola Crenulata H.by GC-MS was studied.The crenulatin was extracted with water,and then separated with macroporous resin column,and analyzed with GC-MS.The major characteristic ion fragments (m/z) was 69.When the selected ion mode (GC/MS-SIM) was chosen as the quantitative method,the recovery was 93.70%～98.58%.The variable coefficient was 1.23%～2.94%.The linear range was 0.10～0.50 mg/mL and a detection limit(3 S/N)was 0.01 mg/mL. This method is precise and selective,wide liner range,little interference.It is suitable for the determination of crenulatin in Rhodiola Crenulata H. |
| Keywords: | crenulatin Rhodiola Crenulata H.,determination gas chromatography-mass spectrometry(GCMS) |
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