| 桑黄菌胞内多糖的理化性质和体外抗氧化活性 |
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| 引用本文： | 闫景坤,马海乐,祝子坪,郑惠华,杨小明,刘伟民. 桑黄菌胞内多糖的理化性质和体外抗氧化活性[J]. 食品科学, 2012, 33(9): 36-40. DOI: 10.7506/spkx1002-6630-201209008 |
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| 作者姓名： | 闫景坤 马海乐 祝子坪 郑惠华 杨小明 刘伟民 |
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| 作者单位： | 1.江苏大学食品与生物工程学院，江苏省农产品物理加工重点实验室2.江苏安惠生物科技有限公司 |
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| 基金项目： | 江苏高校优势学科建设工程资助项目;江苏省农产品物理加工重点实验室开放基金项目(JAPP2010-7);江苏大学校基金资助项目(105DG129) |
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| 摘 要： | 研究液体发酵桑黄菌胞内多糖的理化性质和体外抗氧化活性。结果表明：经DEAE-52纤维素离子交换柱层析分离分级得到多个级分，以较大分子质量的中性多糖为主。凝胶渗透色谱分析表明桑黄菌胞内多糖的重均分子质量范围为5.7×103～6.1×106D，由葡萄糖、甘露糖和半乳糖组成，其分子物质的量比为15:4:1，多糖含量为41%，特性黏度为12mL/g。通过体外抗氧化模型，发现桑黄菌胞内多糖均能较好的清除 ·OH、O2－ ·和螯合Fe2+，且对O2－ ·具有明显的清除作用。
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| 关 键 词： | 桑黄 胞内多糖 理化性质 体外抗氧化 |
Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius |
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| YAN Jing-kun,MA Hai-le,ZHU Zi-ping,ZHENG Hui-hua,YANG Xiao-ming,LIU Wei-min. Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius[J]. Food Science, 2012, 33(9): 36-40. DOI: 10.7506/spkx1002-6630-201209008 |
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| Authors: | YAN Jing-kun MA Hai-le ZHU Zi-ping ZHENG Hui-hua YANG Xiao-ming LIU Wei-min |
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| Affiliation: | (1. Jiangsu Key Laboratory of Physical Processing of Agricultural Product, School of Food and Biological Engineering, JiangsuUniversity, Zhenjiang 212013, China ；2. Jiangsu Alphay Biological Technology Co. Ltd., Nantong 226009, China) |
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| Abstract: | In this study, physicochemical properties and antioxidant activity of intracellular polysaccharides (IPs) isolated from liquid mycelial culture of Phellinus igniarius were analyzed. Crude IPs extract was separated into 8 fractions by DEAE-52 cellulose anion-exchange column chromatography, most of which were high molecular weight neutral polysaccharides. Gel permeation chromatographic (GPC) analysis showed that the average molecular weight (MW) of IPs ranged from 5.7 × 103 D to 6.1 × 106 D. The monosaccharide composition consisted of glucose, mannose, and galactose at a molar ratio of 15:4:1, the polysaccharide content in crude IPs extract was 41% and its intrinsic viscosity was 12 mL/g. IPs from Phellinus igniarius mycelia could effectively scavenge hydroxyl and superoxide anion free radicals and chelate Fe2+. Furthermore, more obvious scavenging activity was observed against superoxide anion free radicals than hydroxyl free radicals. |
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| Keywords: | Phellinus igniarius intracellular polysaccharides(IPs) physico-chemical properties antioxidant activity in vitro |
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