固相萃取-高效液相色谱法测定饮料中的9种合成着色剂

本文介绍了一种运用固相萃取-高效液相色谱技术同时快速检测饮料中柠檬黄、日落黄、新红、苋菜红、胭脂红、酸性红、诱惑红、亮蓝、靛蓝等9种合成着色剂含量的方法。样品经固相萃取柱提取后,采用Purospher~? STARLP RP-18 endcapped色谱柱(4.6 mm×250 mm×5μm)分离,用20 mmol/L乙酸铵水溶液和乙腈体系作为流动相进行梯度洗脱,采用二极管阵列检测器,在200-700 nm波长下进行扫描,254 nm波长下进行检测。在此实验条件下,9种合成着色剂可在25 min内有效分离,平均回收率为77.3-98.4%,相对标准偏差为0.14%-3.81%,检出限为4.12-4.92 mg/kg。建立的方法可实现对饮料中9种合成着色剂的同时检测,具有快速、准确、灵敏的特点。

Simultaneous Determination of Nine Synthetic Colorants in Beverages by High Performance Liquid Chromatography with Solid Phase Extraction

In this paper,a high performance liquid chromatography with solid phase extraction method was built to determine 9 synthetic colorants, including tartrazine, sunset yellow, new red, amaranth, ponceau 4R,carmoisine,allura red,brilliant blue and indigotine in beverages.After a solid phase extraction, 9 synthetic colorants were separated by Purospher? STARLP RP-18 endcapped(4.6 mm×250 mm×5 μm) usingammonium acetate(20 mmol/L)as mobile phase A, acetonitrile as mobile phase B in gradient elution. These synthetic colorants were detected by diode array detector in the wavelength range from 200-700 nm with detection wave length at 254 nm. Underthe optimal experimental conditions,9 synthetic colorants were rapidly separated within 25 minutes. The average recoveries were in the range of 77.3% to 98.4% and the relative standard deviations(RSDs) were 0.14%-3.81%. The limits of detection(LODs) of 9 synthetic colorants were 4.12-4.92 mg/kg. The established method is rapid,accurate and sensitive,which is suitable for simultaneous detection of 9 synthetic colorants in beverages.