| 地高辛标记HBV DNA探针的应用 |
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| 引用本文： | 董伟,肖卫华,王玲,贾国富,翟立炜,于万龙,徐德启.地高辛标记HBV DNA探针的应用[J].粉末涂料与涂装,1992(1). |
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| 作者姓名： | 董伟 肖卫华 王玲 贾国富 翟立炜 于万龙 徐德启 |
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| 作者单位： | 卫生部长春生物制品研究所，卫生部长春生物制品研究所，卫生部长春生物制品研究所，卫生部长春生物制品研究所，卫生部长春生物制品研究所，卫生部长春生物制品研究所，卫生部长春生物制品研究所 长春 130062，长春 130062，长春 130062，长春 130062，长春 130062，长春 130062，长春 130062 |
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| 摘 要： | 按随机引物法以地高辛标记HBV DNA探针,与按缺口翻译法以~(32)P标记的探针进行了比较。这两种探针与乙肝血源疫苗及患者血清标本中HBV DNA进行杂交,结果两种探针均可要出1pg同源序列DNA,而地高辛标记的探针,在0.1Pg水平仍可呈现弱着色反应。该探针在一20℃储存6个月,其敏感性不减弱。这种探针在常规诊断实验室更易推广使用。
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| 关 键 词： | HBV DNA杂交 地高辛 |
Application of HBV DNA probe labelled with digoxigenin |
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| Dong Wei et al Changchun Institute of Biological Products,Changchun.Application of HBV DNA probe labelled with digoxigenin[J].Chinese Journal of Biologicals,1992(1). |
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| Authors: | Dong Wei Changchun Institute of Biological Products Changchun |
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| Abstract: | The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP, and compared with ~(32)P-labelled probe by nick translation. The two probes were used to detect HBV DNA in plasma- derived HB Vaccine and sera of clinical patients by using Dot-blot hybridization. The results showed that the sensitivity of the two probes were comparable.Both of them could detect 1 pg of homologous DNA, but for the 0.1 pg level of HBV DNA could be detected only with a faint coloured precipitate by digoxigen-labelled probe. The stability experiments of the probe demonstrated that the sensitivity did not decreese after 6 months at -20℃. The non-isotope labelled probe is more readily applicable in routine diagnostic laboratories. |
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| Keywords: | HBV DNA hybridization Digoxigenin |
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