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| 多表位DNA壳聚糖微球疫苗的体液免疫应答 | |
| 引用本文： | 程玉兴,朱高红,瞿素,兰芸,胡云章,胡凝珠. 多表位DNA壳聚糖微球疫苗的体液免疫应答[J]. 中国生物制品学杂志, 2007, 20(6): 429-431 |
| 作者姓名： | 程玉兴 朱高红 瞿素 兰芸 胡云章 胡凝珠 |
| 作者单位： | 中国医学科学院中国协和医科大学医学生物学研究所疫苗研究室 (昆明650118)(程玉兴，瞿素，兰芸，胡云章，胡凝珠)，昆明医学院附一院核医学科 (昆明650032)(朱高红) |
| 摘 要： | 目的研究多表位DNA壳聚糖微球疫苗的体液免疫应答。方法制备多表位DNA壳聚糖微球疫苗pcD-NA3.1-HME-3C3d,经鼻腔免疫小鼠,蛋白检测微孔试剂盒检测小鼠特异性IgG抗体水平。结果经免疫的小鼠均能产生针对各表位的特异性IgG抗体,DNA壳聚糖微球疫苗的抗体水平明显高于DNA疫苗。结论壳聚糖微球疫苗投递系统可提高多表位DNA疫苗的免疫应答效果。 |
| 关 键 词： | 多表位DNA疫苗 体液免疫应答 壳聚糖微球 |
| 文章编号： | 1004-5503（2007）06-429-03 |
| 收稿时间： | 2006-09-15 |
| 修稿时间： | 2006-09-15 |
Humoral Immune Response Induced by Polytope DNA Vaccine in Chitosan based Microspheres |
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| CHENG Yu-xing ,ZHU Gao-hong, QU Su, et al. Humoral Immune Response Induced by Polytope DNA Vaccine in Chitosan based Microspheres[J]. Chinese Journal of Bilogicals, 2007, 20(6): 429-431 | |
| Authors: | CHENG Yu-xing ZHU Gao-hong QU Su et al |
| Affiliation: | Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China |
| Abstract: | Objective To study the humoral immune response induced by polytope DNA vaccine in chitosan-based microsphere.Methods Prepare the constructed DNA vaccines pcDNA3.1-HME and pcDNA3.1-HME-3C3d into chitosan-based microspheres and immunize mice intranasally.Determine the specific antibody level induced in mice by micropore kit for protein detection.Results The specific IgGs to various epitopes were induced in immunized mice.However,the IgG level induced by chitosan-based DNA microsphere vaccine was significantly higher than that by DNA vaccine.Conclusion The chitosan-based microsphere delivery system enhanced the humoral immune response induced by polytope DNA vaccine. |
| Keywords: | Polytope DNA vaccine Humoral immune response Chitosan-based microsphere |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |