An improved PCR-based method for site directed mutagenesis using megaprimers |
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| Authors: | J Br?ns-Poulsen NE Petersen M H?rder K Kristiansen |
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| Affiliation: | Department of Biochemistry and Food Chemistry, University of Turku, Finland. |
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| Abstract: | The antimicrobial activity of a combination of lactic acid and whey permeate fermented by a nisin-producing Lactococcus lactis strain was tested by the agar diffusion method using bacteria isolated from fish as test organisms. Lactic acid inhibited all bacterial strains studied, but nisin whey permeate inhibited Gram-positive bacteria only. The combination was more effective than lactic acid alone against Pseudomonas fluorescens and Staphylococcus hominis isolated from fish, and Pseudomonas aeruginosa ATCC9721 and Micrococcus luteus ATCC9341. |
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