| 抗人M型红细胞膜血型糖蛋白A单克隆抗体的理化特性研究 |
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| 引用本文： | 鲍蕾,张建,郑剑玲,韦星呈,张秀梅. 抗人M型红细胞膜血型糖蛋白A单克隆抗体的理化特性研究[J]. 中国生物制品学杂志, 1997, 0(3) |
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| 作者姓名： | 鲍蕾 张建 郑剑玲 韦星呈 张秀梅 |
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| 作者单位： | 辽宁省基础医学研究所!沈阳，110005，辽宁省基础医学研究所!沈阳，110005，辽宁省基础医学研究所!沈阳，110005，辽宁省基础医学研究所!沈阳，110005，辽宁省基础医学研究所!沈阳，110005 |
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| 摘 要： | 本文报告了理化因素对抗人M型红细胞膜血型糖蛋白A单克隆抗体（4E6、4H6、4D8、2C8）活性的影响。在PH7.5～9.5，反应温度20～30℃范围内，只有4E6特异性凝集具有M血型抗原的红细胞，其余各单克隆抗体与N型红细胞有交叉凝集反应。选择pH8.0，20℃适宜的反应条件，用4E6单充隆抗体对516份成人外周血及65份胎儿脐带血进行MN分型，结果与市售MN分型用动物免疫标准血清完全一致。4E6对猪等8种动物红细胞无交叉反应。
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| 关 键 词： | 抗M单克隆抗体 血型糖蛋白A MN血型 理化特性 |
Study on Physicochemical Characters of McAbs to Glycophorin A from Human M Blood Group Red Cell Membrane |
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| Bao Lei, Zhang Jian, Zheng Jianling et al. Study on Physicochemical Characters of McAbs to Glycophorin A from Human M Blood Group Red Cell Membrane[J]. Chinese Journal of Bilogicals, 1997, 0(3) |
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| Authors: | Bao Lei Zhang Jian Zheng Jianling et al |
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| Abstract: | The effect of physicochemical factors on the activities of McAbs (4E6, 4H6,4D8, 2C8)to glycophorin A from human M blood group red cell membrane was reported. Only 4E, specifically agglutinated with the M blood group antigen on the surface of red cell membrane at pH7. 5~9. 5 and at 20~30℃. The rest of McAbs showed cross agglutination reactions with N blood group red cells under the same conditions. The M, N blood group typing tests of 516 samples of adult peripheral blood and 65 samples of fetal cord blood were performed using 4E6 at pH8. 0 and at 20℃. The results were fully coincident with those using commercial animal immune serum standards for MN blood group typing. In addition, 4E6showed no cross reaction with 8 kinds of animal red cells. |
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| Keywords: | McAb to M blood group Glycophorin A MN blood group Physicochemical character |
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