Mass spectrometric analysis of the fatty acids and nonsaponifiable lipids ofEimeria tenella oocysts |
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Authors: | R M Weppelman W J A Vandenheuvel C C Wang |
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Affiliation: | (1) Merck Sharp & Dohme Research Laboratories, 07065 Rahway, New Jersey |
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Abstract: | The fatty acids and nonsaponifiable lipids ofEimeria tenella oocysts were analyzed by gas liquid chromatography and combined gas liquid chromatographymass spectrometry. The fatty acids
detected were identified as C14∶0, C16∶0, C16∶1, C18∶0, C18∶1, and C18∶2. Though the wt of the fatty acid fraction decreased during sporulation from 91 μg per 106 oocysts to 47 μg per 106 oocysts, the relative amounts of these fatty acids did not change appreciably. The nonsaponifiable lipids ofE. tenella consisted of cholesterol and unbranched primary alcohols of 22, 24, 26, 28, 30, and 32 carbons. Mass fragmentography demonstrated
that each species of alcohol consisted of saturated and monounsaturated derivatives. Trimethylsilyl ethers of fatty alcohols
were found to offer several important advantages over free alcohols for mass spectrometric characterization. Before sporulation,
most fatty alcohols were in the oocyst wall. During sporulation, the wt of the nonsaponifiable lipids increased from 16 μg
per 106 oocysts of 44 μg per 106 oocysts due largely to synthesis of C24 and C26 alcohols. The newly synthesized fatty alcohols were not deposited in the oocyst wall. |
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