Real-time detection of hyperosmotic stress response in optically trapped single yeast cells using Raman microspectroscopy |
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Authors: | Singh Gajendra P Creely Caitriona M Volpe Giovanni Grötsch Helga Petrov Dmitri |
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Affiliation: | ICFO-Institut de Ciències Fotoniques, Jordi Girona 29, Nexus II, 08034 Barcelona, Spain. |
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Abstract: | Living cells survive environmentally stressful conditions by initiating a stress response. We monitored changes in the Raman spectra of optically trapped Saccharomyces cerevisiae yeast cell under normal, heat-treated, and hyperosmotic stress conditions. It is shown that when glucose was used to exert hyperosmotic stress, two chemical substances-glycerol and ethanol-can be monitored in real time in a single cell. |
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