Studies on agaroses; 1. Specific refractive index increments in dimethyl sulfoxide and in water at various wavelengths and temperatures
Affiliation:
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovský Sq. 2, 162 06, Prague 6, Czech Republic
Biochemical Separation Centre, Biomedical Centre, Uppsala University, Box 577, S-751 23, Uppsala, Sweden
Abstract:
Specific refractive index increments of three agarose samples in dimethyl sulfoxide and in water were measured at 436, 546 and 633 nm, and at several temperatures. The wavelength and temperature dependences of the increments were determined, simultaneously correlated using the surface regression method, and presented in a form suitable for tabulation. The influence of differences in the structure of agaroses from various algal sources on the specific refractive index increment is discussed.