Measurement oftrans and other isomeric unsaturated fatty acids in butterand margarine |
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Authors: | L M Smith W L Dunkley A Franke T Dairiki |
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Affiliation: | (1) Department of Food Science and Technology, University of California, 95616 Davis, California |
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Abstract: | A procedure is described for gas liquid chromatographic determination of cis andtrans isomers of unsaturated fatty acids after fractionation of the saturated, monenoic, dienoic, and polyenoic fatty acid methyl
esters by argentation thin layer chromatography. To test its reliability, the procedure was used for quantitative measurement
of transisomers of unsaturated fatty acids in a known mixture of simple triglycerides containing saturated fatty acids from
4:0 to 24:0 andcis andtrans isomers of 14:1. 16:1, 18:1, and 18:2. Results of the analyses of five margarine and five butter samples are presented, together
with results of infrared spectrophotometric analyses fortrans fatty acid concentrations, ultraviolet spectrophotometric analyses for conjugated fatty acid concentrations, and enzymatic
analyses forcis-cis-methylene interrupted fatty acid concentrations. The combined argentation thin layer and gas Chromatographic procedure is
suitable for determination of the principal fatty acids in complex food lipids such as milk fat. |
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