| 五种野生青海食用菌提取物的抗氧化活性研究 |
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| 引用本文： | 李宏吉,焦迎春,范琳琳,董亚晨,陈启和.五种野生青海食用菌提取物的抗氧化活性研究[J].现代食品科技,2014,30(11):188-193. |
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| 作者姓名： | 李宏吉 焦迎春 范琳琳 董亚晨 陈启和 |
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| 作者单位： | 浙江大学食品科学与营养系，浙江杭州 310058;（1.浙江大学食品科学与营养系，浙江杭州 310058）（2.青海大学农牧学院，青海西宁 810016）;浙江大学食品科学与营养系，浙江杭州 310058;浙江大学食品科学与营养系，浙江杭州 310058;浙江大学食品科学与营养系，浙江杭州 310058 |
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| 基金项目： | 浙江省自然科学基金项目（LY12C20009；LY13H300003)；教育部新世纪优秀人才支持计划（NCET-12-0482） |
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| 摘 要： | 本文综合分析了草原白蘑菇1号Agaricus bisporus(Lange)Sing.、毛头鬼伞Coprinus comatus(Muell.:Fr.)S.F.Gray.、墨汁鬼伞Copinus atramentarius(Bull.)Fr.、长根菇Collybia radi-cata(Relh.ex Fr.)Quel.和翘林丝盖伞Inocybe calamistrata(Fr.)Gill.五种来自青海的野生食用真菌的抗氧化活性及相关成分。翘林丝盖伞的两种提取物均表现出了最强的DPPH自由基清除能力,其甲醇提取物和乙醇提取物的EC50值分别为0.90±0.06 mg/m L和0.85±0.14 mg/m L;长根菇甲醇提取物(0.45±0.01 mg/m L)和乙醇提取物(0.78±0.01mg/m L)展现出了最强的铁离子螯合能力;翘林丝盖伞甲醇提取物(0.26±0.002 mmol Trolox equivalents/g)和55%乙醇提取物(0.24±0.003mmol Trolox equivalents/g)具有最高的Trolox等价摩尔含量。墨汁鬼伞甲醇提取物中抗氧化活性成分-总酚的含量(19.04±0.30 mg gallic acid equivalents/g)显著高于其他四种提取物(P0.05);翘林丝盖伞乙醇提取物的总酚含量(11.41±0.09 mg gallic acid equivalents/g)显著高于其他四种提取物(P0.05)。五种野生食用真菌均具有显著的抗氧化活性,总体上来说,翘林丝盖伞的抗氧化活性更强。
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| 关 键 词： | 青海野生食用真菌 抗氧化活性 总酚含量 |
| 收稿时间： | 2014/4/18 0:00:00 |
Antioxidant Activities of Five Wild Edible Mushrooms Extracts From Qinghai |
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| LI Hong-ji,JIAO Ying-chun,FAN Lin-lin,DONG Ya-chen and CHEN Qi-he.Antioxidant Activities of Five Wild Edible Mushrooms Extracts From Qinghai[J].Modern Food Science & Technology,2014,30(11):188-193. |
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| Authors: | LI Hong-ji JIAO Ying-chun FAN Lin-lin DONG Ya-chen and CHEN Qi-he |
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| Affiliation: | Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China;(1.Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China)
(2.Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China);Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China;Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China;Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | The aim of the present study was to comprehensively analyze the antioxidant activities and relevant components of five wild edible mushrooms: Agaricus bisporus (Lange) Sing., Coprinus comatus (Muell: Fr.) S. F. Gray., Coprinus atramentarius (Bull.) Fr., Collybia radicata (Relh. ex Fr.) Quel., and Inocybe calamistrata (Fr.) Gill. from Qinghai province. The two extracts of I. calamistrata showed the strongest 2,2-diphenyl-1-picryhydrazyl (DPPH) scavenging capacity, and the EC50 values of its methanolic crude extract (MCE) and ethanolic crude extract (ECE) were 0.90 ± 0.06 mg/mL and 0.85 ± 0.14 mg/mL, respectively. The MCE (0.45 ± 0.01 mg/mL) and ECE (0.78 ± 0.01 mg/mL) of C. radicata showed the strongest metal chelating capacity. Both the MCE (0.26 ± 0.002 mmol Trolox equivalents/g) and ECE (0.24 ± 0.003 mmol Trolox equivalents/g) of I. calamistrata possessed the highest micromolar Trolox equivalent per gram. The total content of phenols (active antioxidant components) was significantly higher in the MCE of C. atramentarius (19.04 ± 0.30 mg gallic acid equivalents/g) than of the other four extracts (P < 0.05). Similarly, the total phenolic content was significantly higher in the ECE of I. calamistrata than of the other four extracts (P < 0.05). All five edible mushrooms showed marked antioxidant capacities; I. calamistrata showed the strongest antioxidant activity. |
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| Keywords: | wild edible mushrooms from Qinghai antioxidant activity total phenolic content |
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