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| 贵州烟草上一株PVY坏死株系全序列测定与分析 | |
| 引用本文： | 张帅,杨金广,王凤龙,钱玉梅,申莉莉,王永.贵州烟草上一株PVY坏死株系全序列测定与分析[J].中国烟草科学,2011,32(1):47-51. |
| 作者姓名： | 张帅 杨金广 王凤龙 钱玉梅 申莉莉 王永 |
| 作者单位： | 1. 烟草行业病虫害监测与综合治理重点实验室,农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101;中国农业科学院研究生院,北京,100081 2. 烟草行业病虫害监测与综合治理重点实验室,农业部烟草类作物质量控制重点开放实验室,中国农业科学院烟草研究所,青岛,266101 3. 山东中烟工业有限责任公司,济南,250100 |
| 摘 要： | 2007年从贵州省贵定烟区采集一株呈典型马铃薯Y病毒坏死株系(Potato virus Y vein necrosis strain,PVYN)的烟草病毒病样,经PVY鉴别寄主枸杞(Lycium barbarum L.)单斑分离纯化后,于枯斑三生烟(Samsun NN)株上保存,得到PVY分离物Guiding-3。以GenBank中已有的PVY全序列为基础,设计了3对PVY特异引物。通过总RNA提取、RT-PCR及序列测定,发现所测得的PVY分离物Guiding-3全长基因组序列(NCBI登录号为HM590405)包括9 699个碱基,整条序列仅包含一个由9 186个碱基组成的开放读码框(ORF),编码一条包含3 061个氨基酸的多聚蛋白。利用MEGA软件对所测得的PVY全序列与GenBank中已有的PVY全序列进行系统进化分析,发现Guiding-3分离物的序列与PVYN、PVYNTN、PVYN:O、PVYO、PVYNNP株系的一致性分别为98%、97%、94%、92%、85%,在已有的PVY株系中,Guiding-3分离物与PVYN株系具有较高的进化亲缘关系。另外,还对PVY的分子变异进行了初步讨论。 |
| 关 键 词： | 烟草 马铃薯Y病毒 全序列分析 |
Sequencing and Analysis of PVYN Strain in Tobacco from Guizhou Province |
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| ZHANG Shuai,YANG Jinguang,WANG Fenglong,QIAN Yumei,SHEN Lili,WANG Yong.Sequencing and Analysis of PVYN Strain in Tobacco from Guizhou Province[J].Chinese Tobacco Science,2011,32(1):47-51. | |
| Authors: | ZHANG Shuai YANG Jinguang WANG Fenglong QIAN Yumei SHEN Lili WANG Yong |
| Affiliation: | ZHANG Shuai1,2,YANG Jinguang1,WANG Fenglong1*,QIAN Yumei1,SHEN Lili1,WANG Yong3(1.Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management,State Tobacco Monopoly Bureau,Key Laboratory for Tobacco Quality Control,Ministry of Agriculture,Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China,2.Graduate School of CAAS,Beijing 100081,3.China Tobacco Shandong Industrial Co.,Ltd.,Jinan 250100,China) |
| Abstract: | One tobacco virus disease sample was collected from Guiding tobacco regions in Guizhou province at 2007 which showed typical PVYN strain symptom.After three successive inoculations on Lycium barbarum L.as necrotic lesion host of PVY,and PVY was purified and maintained on Nicotiana tabacum Samsun NN plant,and named Guiding-3.Three pairs of specific primers based on genome sequences of PVY registered in GeneBank were designed.After total RNA extraction,RT-PCR,product sequencing,the sequence of PVY Gduiing-3 i... |
| Keywords: | tobacco Potato virus Y complete sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |