| 茶叶多糖的提取纯化及其单糖组分的鉴定 |
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| 引用本文： | 刘咏,成战胜. 茶叶多糖的提取纯化及其单糖组分的鉴定[J]. 食品与发酵工业, 2005, 31(6): 134-136 |
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| 作者姓名： | 刘咏 成战胜 |
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| 作者单位： | 1. 合肥工业大学生物与食品工程学院,合肥,230009
2. 焦作大学化学工程系,焦作,454003 |
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| 摘 要： | 研究了用水煮醇沉法提取茶叶多糖,用savage法除蛋白,苯酚-硫酸法测多糖含量,通过正交试验确定茶叶多糖的最佳提取条件;粗多糖经Sephadex-100柱层析纯化,得到精制茶叶多糖;薄层层析法鉴定单糖组分。结果表明:最佳提取条件为95℃,料液比1∶20(g:mL),提取时间2h,提取3次,茶叶多糖含量为35.92mg/g。茶叶多糖中含有2种不同的多糖,水解得到单糖D-半乳糖、D-甘露糖、D-葡萄糖、L-阿拉伯糖等。
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| 关 键 词： | 茶叶 多糖 提取 正交试验 纯化 |
| 修稿时间： | 2004-11-22 |
The Study on Extraction and Purification of Tea Polysaccharides |
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| Liu Yong,Chen Zhansheng. The Study on Extraction and Purification of Tea Polysaccharides[J]. Food and Fermentation Industries, 2005, 31(6): 134-136 |
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| Authors: | Liu Yong Chen Zhansheng |
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| Affiliation: | Liu Yong~1 Chen Zhansheng~2 1 |
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| Abstract: | A water-soluble polysaccharide was extracted from Tea Leaves by of boiling water then ethanol deposition. deproteinized by Seveg and determined the content of polysaccharides with phenol-sulfuric acid. The optimal condition was determined by the orthogonal experiment. The crude polysaccharides were isolated and purified by chromatographying with Sephadex G-100 column, The results showed that the optimal extraction conditions were 95℃, 2 h, 3 times extraction as well as the ratio 1∶20 (w/v). Under this condition 35.92 mg/g(DW) polysaccharides was obtained. The Tea polysaccharides had two different polysaccharides which contain D-galactose, D-mannose, D-glucose and L-arabinose after hydrolysis. |
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| Keywords: | tea leaves polysaccharides extraction orthogonal experiment purify |
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