高浓度CaCl2溶液对某些肌原纤维蛋白的作用研究

用0、100、200、300mmol/LCaCl2溶液处理肌球蛋白、肌动蛋白和肌原纤维,通过SDS-PAGE和免疫印迹电泳研究100、200、300mmol/L高浓度CaCl2溶液处理下肌球蛋白、肌动蛋白、α-肌动素和肌钙蛋白-T的蛋白降解情况。SDS-PAGE结果显示,肌球蛋白、肌动蛋白在100、200、300mmol/LCaCl2溶液处理下1、7d时的电泳结果与对照组相比没有显著差异。免疫电泳结果显示,100、200、300mmol/L高浓度CaCl2溶液处理后,α-肌动素和肌钙蛋白-T与对照组相比发生显著降解,随着浓度升高,降解程度加大,在300mmol/L浓度时,α-肌动素条带密度显著降低,肌钙蛋白-T也裂解为30kD以下的更小片段。

Study on Effects of High Concentration of Calcium Chloride Solution on Myofibrillar Proteins

Myosin, actin, and myofibrils were incubated in 0, 100, 200, 300 mmol/L calcium chloride solutions respectively, and degradations were determined by SDS-PAGE and immunoblotting. Results showed that there were no obvious differences when myosin and actin were treated with 100, 200, 300 mmol/L concentration compared to 0 concentration.α-actinin and troponin-T were found that degradation occurred incubated in 100, 200, 300 mmol/L calcium chloride solutions, and degradation increased with concentration of calcium chloride increasing. Incubated in 300 mmol/L calcium chloride solutions, most of α-actinin degraded, and troponin-T degraded to smaller fragment less than 30 kD.