| 实时荧光定量PCR技术在食源性致病菌检测中的应用 |
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| 引用本文： | 李敏,綦国红. 实时荧光定量PCR技术在食源性致病菌检测中的应用[J]. 中国食物与营养, 2014, 0(8): 13-17 |
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| 作者姓名： | 李敏 綦国红 |
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| 作者单位： | 中国药科大学药学院,南京210009 |
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| 基金项目： | 国家自然科学基金青年项目（项目编号：30700627）;江苏省级大学生创新创业训练计划项目（项目编号：J1030830）. |
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| 摘 要： | 实时荧光定量PCR技术作为一种高效的微生物定量检测法,在食品工业中具有广泛的应用前景。本文综述了实时荧光定量PCR技术检测原理,及其在食源性致病菌如金黄色葡萄球菌、大肠杆菌O157∶H7、沙门氏菌和副溶血性弧菌检测中的应用。
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| 关 键 词： | 实时荧光定量PCR技术 食源性致病菌检测 微生物检测 定量检测 |
Application of Real-time PCR in the Detection of Foodborne Pathogens |
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| LI Min,QI Guo-hong. Application of Real-time PCR in the Detection of Foodborne Pathogens[J]. Food and Nutrition in China, 2014, 0(8): 13-17 |
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| Authors: | LI Min QI Guo-hong |
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| Affiliation: | (College of Pharmacy, China Pharmaceutical University, Nanjing 210009, China) |
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| Abstract: | The Real-time PCR, which is an efficient method of quantitative detection of microorganisms, has abroad application prospects in food industry. This article introduced the principle of Real-time PCR, as well as its application in detection of foodborne pathogens, such as Staphylococcus aureus, Escherichia coli O157 ：H7, Salmonella and Vibrio parahaemolyticus. |
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| Keywords: | Real-time PCR detection of foodborne pathogens detection of microorganisms quantitative detection |
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