| 油茶饼中茶皂素的定量分析 |
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| 引用本文： | 谭搏,曹福祥,赵莹. 油茶饼中茶皂素的定量分析[J]. 精细化工中间体, 2009, 39(2): 67-69 |
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| 作者姓名： | 谭搏 曹福祥 赵莹 |
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| 作者单位： | 中南林业科技大学,湖南,长沙,410004;中南林业科技大学,湖南,长沙,410004;中南林业科技大学,湖南,长沙,410004 |
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| 摘 要： | 研究了用反相高效液相色谱法（HPLC）测定茶皂素含量的方法，以VP-ODS（250mm×4.6mm，C18，5μm）作分析柱，以甲醇-水-（v：v=9：1）作流动相，流速控制在1．0mL／min，检测波长280nm，以质量浓度为横坐标，峰面积为纵坐标，得到工作曲线为Y=3．02×10^6X-1．16×10^4，线性相关系数为0．9995。回收率为97．02％～102．82％。通过反相高效液相色谱法测定茶籽饼中粗茶皂素的含量为11．89％。
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| 关 键 词： | 反向高效液相色谱 茶皂素 油茶 |
Quantitative Analysis of Tea Saponin in Camellia Cake |
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| TAN Bo,CAO Fu-xiang,ZHAO Ying. Quantitative Analysis of Tea Saponin in Camellia Cake[J]. Fine Chemical Intermediates, 2009, 39(2): 67-69 |
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| Authors: | TAN Bo CAO Fu-xiang ZHAO Ying |
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| Affiliation: | South Central University of Forestry and Technology;Changsha 410004;China |
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| Abstract: | A reverse-phase high performance liquid chromatography(HPLC) method for determination of tea saponin extracted from tea-seed pancake with Soxhlet was studied.With the column VP-ODS(250 mm×4.6 mm,C18,5 μm) and mobile phase methanol-water(v:v=9:1) at flow-rate of 1.0 mL / min,a linear equation was obtained as Y=3.02×106X-1.16×104,where Y represents peak area and X represents mass concentration.Linear correlation coefficient r was 0.999 5.Under these conditions,the recovery rate of tea saponin was 97.02% ～ 102.82%. |
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| Keywords: | reverse-phase high performance liquid chromatography tea saponin camellia oleifera |
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