Quantitation of type B-trichothecene mycotoxins in foods and feeds by a multiple stable isotope dilution assay |
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Authors: | Stefan Asam Michael Rychlik |
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Affiliation: | 1. Lehrstuhl für Lebensmittelchemie der Technischen, Universit?t München, Lichtenbergstr. 4, 85748, Garching, Germany
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Abstract: | For quantitation of the trichothecene mycotoxins deoxynivalenol (DON), 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol and
4-acetylnivalenol (Fusarenon X), stable isotope dilution assays (SIDAs) were developed by using analogues labelled with carbon-13
as the internal standards. The SIDAs presented here are the first ones reported for type B-trichothecenes. The synthesis of
labelled acetyl derivatives was accomplished by 13C2]-acetylation of unlabelled trichothecenes and subsequent hydrolysis of the products. The mycotoxins were quantified in foods
by LC tandem MS after cleanup on multifunctional columns. The method revealed an excellent sensitivity with low detection
and quantification limits for all mycotoxins in the low μg/kg range, and good precision in inter-assay stu- dies. The analysis
of a certified reference material (maize flour) resulted in a low bias of 2.1% from the certified value for DON and verified
excellent trueness of the new method. To prove the suitability of SIDA, a number of cereals and cereal products were analysed
and revealed DON contents ranging from 2 to 300 μg/kg. Acetylated derivatives occurred in lower contents and mainly in corn
products. |
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Keywords: | 3-Acetyldeoxynivalenol 15-Acetyldeoxynivalenol Deoxynivalenol Fusarenon X LC– MS/MS Stable isotope dilution assay Trichothecenes |
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