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| 克菌丹诱导人支气管上皮细胞转化 | |
| 引用本文： | 许建宁,董琳,王全凯,胡洁,李学峰. 克菌丹诱导人支气管上皮细胞转化[J]. 农药, 2011, 50(4) |
| 作者姓名： | 许建宁 董琳 王全凯 胡洁 李学峰 |
| 作者单位： | 1. 中国农业大学理学院,北京,100094;中国疾病预防控制中心职业卫生与中毒控制所,化学污染与健康安全重点实验室,北京,100050 2. 中国疾病预防控制中心职业卫生与中毒控制所,化学污染与健康安全重点实验室,北京,100050 3. 中国农业大学理学院,北京,100094 |
| 摘 要： | [方法]16HBE细胞经0.0625～1.0 mg/L剂量的克菌丹染毒,通过观察染毒细胞形态及恶性表型鉴定,检测克菌丹诱导16HBE细胞的转化.[结果]克菌丹转化第30代细胞生长速度增快,对conA凝集敏感性增强,失去贴壁生长依赖性,电镜下可见明显的形态学改变,裸鼠体内形成肿块为鳞状上皮细胞癌.[结论]克菌丹可直接诱导16HBE细胞发生恶性转化,该模型的建立为克菌丹对健康损伤效应研究及其评价奠定了基础. |
| 关 键 词： | 克菌丹 人支气管上皮细胞 细胞转化 |
Transformation of Human Bronchial Epithelial Cells Induced by Captan |
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| XU Jian-ning,DONG Lin,WANG Quan-kai,HU Jie,LI Xue-feng. Transformation of Human Bronchial Epithelial Cells Induced by Captan[J]. Pesticides, 2011, 50(4) | |
| Authors: | XU Jian-ning DONG Lin WANG Quan-kai HU Jie LI Xue-feng |
| Affiliation: | XU Jian-ning1,2,DONG Lin2,WANG Quan-kai2,HU Jie2,LI Xue-feng1(1.college of science,china Agricultural University,Beijing 100094,china,2.chinese center for Disease control and Prevention National institute of occupational health and Poison control,Key laboratory of chemical safety and health,chinese center for Disease control and Prevention,Beijing 100050,china) |
| Abstract: | [Methods] 16hBe cell was treated with captan at the concentration from 0.0625 to 1.0 mg/l and observed biological characteristics to ensure whether cell malignant transformation occurred.[Results] The 30th generation of 16hBe treated with captan grew faster,while its sensibility to conA and ability of growing in semi-solid agar also increased.The morphological change of transformed cells could be founded under scanning electron microscope and the pathology suggested that tumor formed in nude mice was squamo... |
| Keywords: | captan human bronchial epithelial cell cell transformation |
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