产芽孢梭菌纤溶酶的纯化及特性 |
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引用本文: | 赵红,程洁,陆俭,孟筱琦. 产芽孢梭菌纤溶酶的纯化及特性[J]. 中国生物制品学杂志, 2002, 15(1): 27-31 |
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作者姓名: | 赵红 程洁 陆俭 孟筱琦 |
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作者单位: | 赵红(兰州生物制品研究所,兰州,730046) 程洁(兰州生物制品研究所,兰州,730046) 陆俭(兰州生物制品研究所,兰州,730046) 孟筱琦(兰州生物制品研究所,兰州,730046) |
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摘 要: | 目的 从产芽孢梭菌的培养上清中分离纯化出具有纤溶活性的蛋白质。方法 以产芽孢梭菌的培养上清为材料,经过滤、硫酸铵盐析、离子交换层析纯化纤溶酶,并对其理化特性和纤溶活性进行鉴定。结果 所分离纯化的纤溶酶相对分子质量为67000,系由相对分子质量45000和20000的2个亚基组成,具有较强的溶解纤维蛋白的作用。此酶属丝氨酸蛋白酶类,胰蛋白酶类。结论 为纤溶酶的开发和应用提供了依据。
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关 键 词: | 产芽孢梭菌 纤溶酶 |
修稿时间: | 2001-05-09 |
Purification and Characterization of Fibrinolytic Enzyme of C.Sporogenes Strain |
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Abstract: | Abstract : Objective To purify the protease with fibrinolytic activity from the culture supernatant of Clostridium sporogenes . Methods Fibrinolytic enzyme was purified from the culture supernatant of Clostridi-um Sporogenes by filtration, precipitation with ammonium sulfate and ion - exchange chromatcgraphy, and the physicochemical property and fibrinolytic activity of it were characterized. Results The purified fibrinolytic enzyme, with a relative molecular weight of 67000, consisted of two subunits with the relative molecular weights of 45000 and 20000 respectively. It showed strong fibrinolytic activity. The enzyme was a kind of serine protease with trypsin - like activity. Conclusion The study provides a reliable basis for the development and application of fibrinolytic enzyme. |
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Keywords: | C. sporogenes Fibrinolytic enzyme |
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