Interaction of plasma high density lipoprotein HDL2b (d 1.063–1.100 g/ml) with single-bilayer liposomes of dimyristoylphosphatidylcholine |
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Authors: | Alex V Nichols Elaine L Gong Trudy M Forte Patricia J Blanche |
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Affiliation: | (1) Donner Laboratory, Lawrence Berkeley Laboratory, University of California, 94720 Berkeley, CA |
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Abstract: | Incubation of a major subfraction, HDL2b (d 1.063–1.100 g/ml), of human plasma high density lipoproteins, HDL (d 1.063–1.21 g/ml), with single-bilayer liposomes of
dimyristoylphosphatidylcholine (DMPC) resulted in uptake of DMPC by the HDL2b and dissociation of lipid-free apolipoprotein A-I (apoA-I). In the presence of excess DMPC, the dissociated apoA-I was also
incorporated with DMPC into discoidal complexes. Preliminary studies with model apoA-I-DMPC complexes indicated that they
also can interact with native HDL2b with the resultant transfer of their DMPC to HDL2b and the concomitant release of their apoA-I. After interaction of HDL2b with DMPC liposomes, the DMPC-enriched HDL2b product showed a lower hydrated density and a larger particle size than the control HDL2b. The molecular properties of the lipoprotein product suggest that stabilization of the apoA-I-depleted HDL2b probably occurred via substitution of DMPC for the apoA-I at the HDL2b surface rather than by fusion of the apoA-I-depleted HDL2b. The above interactions of HDL2b with single-bilayer liposomes and discoidal complexes indicate pathways of phospholipid transfer relevant to the possible
role of HDL in the metabolism of lipoprotein surface components in vivo. |
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