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精练酶的酶学特性和复配及降解棉籽壳的效果
引用本文:黄伟,洪枫. 精练酶的酶学特性和复配及降解棉籽壳的效果[J]. 印染, 2012, 38(4): 1-5
作者姓名:黄伟  洪枫
作者单位:东华大学化学化工与生物工程学院微生物工程与工业生物技术研究室,上海,201260
基金项目:国家科技支撑计划(2009BAE88B02);中央高校基本科研业务费专项资金;东华大学生态纺织教育部重点实验室资助
摘    要:以酶学性质接近的纤维素酶、果胶酶和木聚糖酶作为研究对象,测试其最适pH值、最适温度、pH值稳定性和温度稳定性;并以此为基础,研究单一酶和酶复配降解棉籽壳的效果。结果表明,在各自最适反应条件下,木聚糖酶降解棉籽壳的效率最高。酶复配后,在55℃和pH值9的条件下水解10 h,能够有效提高对棉籽壳的降解作用。

关 键 词:精练  木聚糖酶  纤维素酶  果胶酶  复配  棉籽壳

Performance of scouring enzymes and applicationof the complex to degradation of cottonseed hulls
HUANG Wei , HONG Feng. Performance of scouring enzymes and applicationof the complex to degradation of cottonseed hulls[J]. Dyeing and Finishing, 2012, 38(4): 1-5
Authors:HUANG Wei    HONG Feng
Affiliation:Group of Microbiological Engineering and Industrial Biotechnology,College of Chemistry,Chemical Engineering and Biotechnology,Donghua University,Shanghai 201620,Chin
Abstract:Three scouring enzymes including cellulase,pectinase and xylanase are used to remove cottonseed hulls of cotton fabrics.The optimum pH value and temperature,pH stability and temperature stability of the three enzymes are investigated.Degradation of cottonseed hulls with single and combined enzymes is studied.The results show that xylanase demonstrates the best capability on cottonseed hulls destruction among the three enzymes.After using the combination of the three enzymes,the degradation of cottonseed hulls is improved significantly under conditions of hydrolysis at pH 9 and 55 ℃ for 10 h.
Keywords:scouring  xylanase  cellulase  pectinase  combination  cottonseed hull
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