Formation of Anthocyanins from Cells Cultured from Different Parts of Strawberry Plants |
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| Authors: | TSUKASA MORI MIEI SAKURAI JUN-ICHI SHIGETA KUMI YOSHIDA TADAO KONDO |
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| Affiliation: | Authors Mori, Sakurai, and Shigeta are affiliated with the Biotechnology &Environmental Engineering Dept., Research lnstitute, lshikawajima Harima Heavy Industries, Co., Ltd., Yokohama 235, Japan. Author Yoshida is with the Dept. of Food &Nutrition, School of Life Studies, Sugiyama Jogakuen Univ., 17–3 Hoshigaokamotomachi Chikusa, Nagoya 463, Japan. Author Kondo is with the Chemical Instrument Center, Nagoya Univ., Chikusa, Nagoya 464, Japan. |
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| Abstract: | Anthocyanin formation and structural identification were investigated in the cultured strawberry, Fragaria unannssa cv Shikinari. Friable callus tissues were obtained from apical meristems, leafs, and petioles grown on LS agar medium with 2,4-dichlorophenoxyacetic acid and benzyladenine. Using these calli, effects of auxins and cell inoculum size on pigment production were studied in suspension culture. Anthocyanin was produced under 8000 lux after preculture for 3 wk under 800 Iux in suspension culture. The main extracted pigment was identified as a peonidin-3-glucoside and the second major pigment was a cyanidin-3-glucoside by FABMS and NMR. Anthocyanin production was stimulated by 2,4-dichlorophenoxyacetic acid and its yield was about three times that of indole-3-acetic acid. An inoculum rate of 2g (fresh weight)/100 mL LS medium resulted in highest pigment production. The callus derived from the leaf was much higher in anthocyanin production than the others tissues of strawberry. |
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| Keywords: | strawberries callus color anthocyanins cell culture |
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