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| 甜瓜细菌性果斑病病原菌的分离鉴定及16S rDNA序列分析 | |
| 引用本文： | 王新绘,刘晓颖,李 冠. 甜瓜细菌性果斑病病原菌的分离鉴定及16S rDNA序列分析[J]. 食品科学, 2015, 36(19): 186-189. DOI: 10.7506/spkx1002-6630-201519033 |
| 作者姓名： | 王新绘 刘晓颖 李 冠 |
| 作者单位： | 1.新疆大学生命科学与技术学院，新疆 乌鲁木齐 830046；2.新疆大学资源与环境科学学院，新疆 乌鲁木齐 830046 |
| 摘 要： | 从新疆昌吉甜瓜实验田中采集发病甜瓜样品12 份，对甜瓜细菌性果斑病病原菌进行分离纯化，并根据形态观察、致病性测定得到甜瓜细菌性果斑病病原菌1 株。以该菌总DNA为模板，采用细菌16S rDNA通用引物进行聚合酶链式反应扩增，并克隆到pGM-T载体，测序结果表明：克隆的16S rDNA序列长度为1 493 bp，通过GenBank上序列比对搜索工具分析，并构建系统发育树，对该菌株进行鉴定，结果表明该菌属于Acidovorax avenae subsp.citrulli Willems（Aac），即燕麦嗜酸菌西瓜亚种。 |
| 关 键 词： | 甜瓜 细菌性果斑病 燕麦嗜酸菌西瓜亚种 鉴定 |
Isolation,Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon |
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| WANG Xinhui,LIU Xiaoying,LI Guan. Isolation,Identification and 16S rDNA Sequence Analysis of Bacterial Fruit Blotch Pathogen in Melon[J]. Food Science, 2015, 36(19): 186-189. DOI: 10.7506/spkx1002-6630-201519033 | |
| Authors: | WANG Xinhui LIU Xiaoying LI Guan |
| Affiliation: | 1. College of Life Science and Technology, Xinjiang University, Ürümqi 830046, China;2. College of Resource and Environment Sciences, Xinjiang University, Ürümqi 830046, China |
| Abstract: | Twelve fruits of melon with bacterial fruit blotch collected from Changji, Xinjiang were investigated for the isolation and purification of the pathogen. The pathogenic strain was subjected to polymerase chain reaction (PCR) amplification with the bacterial 16S rDNA universal primer using its total DNA as the template and the products were cloned into pGM-T vector. Sequencing analysis revealed that the fragment had a length of 1 493 bp. Basic Local Alignment Search Tool (BLAST) and phylogenetic tree analysis showed that the strain belongs to Acidovorax avenae subsp. citrulli Willems (Aac). |
| Keywords: | melon bacterial fruit blotch Acidovorax avenae subsp. citrulli Willems identification |
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