苦丁茶冬青苦丁茶提取物与3,5-双咖啡酰奎尼酸对肠道微生物体外发酵的影响

制备苦丁茶冬青苦丁茶的水提物和醇提物，并通过HP-20大孔树脂层析和半制备色谱分离纯化得到苦丁茶多酚中含量较高的3,5-双咖啡酰奎尼酸（3,5-dicaffeoylquinic acid，3,5-diCQA）组分。运用体外厌氧发酵和荧光原位杂交技术，探究了苦丁茶提取物和3,5-diCQA对肠道微生物菌群的影响，并测定了发酵体系中短链脂肪酸和乳酸的含量。结果表明，苦丁茶冬青苦丁茶水提物、醇提物和3,5-diCQA能促进双歧杆菌、乳酸菌/肠球菌的生长，同时抑制溶组织梭状菌、普雷沃勒氏菌的生长；与空白对照相比，它们还能促进甲酸、乙酸和丙酸的生成，但对丁酸的合成没有影响。因此，苦丁茶冬青苦丁茶及3,5-diCQA具有一定的调节肠道微生态的作用。

Comparative Study of the Effects on Colonic Microbiota Fermentation in vitro of Extracts from Ilex kudingcha C.J.Tseng and 3,5-Dicaffeoylquinic Acid

We prepared aqueous and ethanolic Kudingcha extracts from Ilex kudingcha C.J. Tseng and 3,5-dicaffeoylquinic
acid, the main fraction of Kudingcha polyphenols, purified from the aqueous extract by HP-20 macroporous resin
chromatography and semi-preparative chromatography. Anaerobic fermentation technology and fluorescence in situ
hybridization were employed to investigate the effects of the Kudingcha extracts and 3,5-diCQA on fermentation
characteristics in vitro of the human gut mictobiota, and the production of short-chain fatty acids and latic acid during the
culture were also examined. The results showed that both Kudingcha extracts and 3,5-diCQA could promote the growth
of Bifidobacterium spp. and Lactobacillus/Enterococcus spp., and inhibit Bacteroides-Prevotella spp. and Clostridium
histolyticum group. The concentrations of formic, acetic and proponic acids in cultures with Kudingch extracts were
relatively higher than those of the control, but there was no difference in butyric acid. The results suggest that both
Kudingcha from I. kudingcha C.J. Tseng and its polyphenol 3,5-diCQA have potential prebiotic-like activity by modulating
the intestinal microbiota.