响应面试验优化苹果渣总三萜超声提取工艺

利用超声波提取技术结合响应面分析法对富士苹果渣中总三萜的提取工艺进行优化。选择苹果渣粉碎粒度、液固比、乙醇体积分数、超声时间和超声温度进行单因素试验，在此基础上采用Plackett-Burman因素筛选设计对影响超声波提取苹果渣总三萜的因素进行试验，筛选出影响显著的因素；然后根据Box-Behnken试验设计原理，选取三因素三水平，以苹果渣总三萜得率为响应值进行响应面分析，确定最优工艺参数。结果表明：Plackett-Burman设计筛选出粉碎粒度、液固比和乙醇体积分数为对苹果渣总三萜得率有显著影响的因素；通过响应面分析，确定苹果渣总三萜最优提取工艺为粉碎粒度100 目、液固比12∶1（mL/g）、提取溶剂为无水乙醇、超声时间20 min、超声温度40 ℃，在此条件下富士苹果渣总三萜的得率为（7.10±0.01）%。

Optimization of Ultrasonic-Assisted Extraction of Total Triterpenoids from Apple Pomace by Response Surface Methodology

Response surface methodology was applied to optimize the ultrasonic-assisted extraction of total triterpenoids
from apple pomace of ‘Fuji’ variety. Particle size of apple pomace, liquid/solid ratio, ethanol concentration, extraction time
and temperature were chosen to carry out single factor experiments, and the former three factors were recognized as the
independent variables with significant influence on the yield of total triterpenoids using Plackett-Burman design. The three
factors were further optimized using response surface methodology based on a three-level, three-variable Box-Behnken
design. By analyzing the response surface plots, the optimal levels for particle size of apple pomace, liquid/solid ratio,
ethanol concentration were determined as 100 mesh, 12:1 (mL/g) and 100%, respectively. Experiments conducted under the
optimized conditions led to an extraction yield of (7.10 ± 0.01)%.