脱落酸对果糖诱导的胰岛素抵抗小鼠的影响

目的：研究脱落酸（abscisic acid，ABA）对果糖诱导的胰岛素抵抗小鼠的影响。方法：将60 只雄性ICR小鼠随机分成6 组，每组10 只，分别为正常对照组、模型组、阳性对照组（盐酸吡格列酮15 mg/（kg•d））和ABA低剂量组（0.75 mg/（kg•d））、ABA中剂量组（1.5 mg/（kg•d））、ABA高剂量组（3 mg/（kg•d）），除正常对照组外，其余各组均给予10%果糖溶液（质量分数）作为饮用水，连续灌胃给药30 d，实验结束后处死小鼠，收集血液后离心，取血清用于测定血糖、胰岛素、血脂水平。取肝脏、肌肉称质量，用于测定肌糖原、肝糖原含量。结果：果糖可显著增加小鼠空腹 血糖及胰岛素水平。给药30 d后，ABA高、中、低剂量组均能显著降低小鼠血糖水平及胰岛素水平，提高小鼠肌糖原、肝糖原含量，ABA中、高剂量组能显著降低小鼠血清中甘油三酯、胆固醇水平，增加高密度脂蛋白胆固醇水平。结论：ABA中、高剂量组能显著提高小鼠对胰岛素的敏感性，较好地干预胰岛素抵抗，且高剂量ABA的胰岛素增敏效果优于盐酸吡格列酮。

Effect of Abscisic Acid in Fructose-Induced Insulin Resistant Mice

Objective: To study the effect of abscisic acid (ABA) in fructose-induced insulin resistant mice. Methods: All male
mice were divided randomly into six groups, including normal control, model, positive control (pioglitazone hydrochloride,
15 mg/(kg·d), and high-dose (3 mg/(kg·d)), medium-dose (1.5 mg/(kg·d)) and low-dose (0.75 mg/(kg·d)) ABA groups
(n =10). Except for the control group, the mice from all the groups were administrated with 10% fructose in drinking water
for 30 consecutive days. At the end of the experiment, all mice were sacrificed. Blood samples were collected to evaluate
glucose, insulin and blood lipid. The contents of muscle glycogen and liver glycogen were determined as well. Results:
Fructose could significantly increase fasting serum glucose concentration and serum insulin concentration. After 30 days
of administration, high-dose, medium-dose and low-dose ABA could significantly reduce serum glucose and insulin
concentrations, and increase muscle glycogen and liver glycogen contents; medium- and high-dose ABA could significantly
reduce serum triglyceride and cholesterol levels, and improve high-density lipoprotein cholesterol level. Conclusion: The
middle-dose and high-dose ABA can significantly improve insulin sensitivity in mice, implying a good intervention effect on
insulin resistance. Its effect is better than that of pioglitazone.