表面等离子共振传感器检测3 种果汁中亚胺硫磷残留

基于亚胺硫磷多克隆抗体，建立苹果汁、桃汁、橙汁中亚胺硫磷残留的表面等离子共振（surface plasmonresonance，SPR）传感器检测方法。采用磷酸盐缓冲液直接稀释样品10 倍后，基本消除基质影响，加标回收率为84.88%～104.69%，变异系数低于6.70%，样品的最低检出限为0.1 μg/L，与高效液相色谱检测结果相关系数为0.992，所建立的SPR检测方法达到了快速准确检测的要求。SPR反应芯片对亚胺硫磷的检测时间为30 min，且在3 d内可反复检测30 次，可有效降低检测成本。通过与同源抗体建立的酶联免疫方法比较发现：SPR方法在检测限、精确度、灵敏度等方面有着一定优势，但对于单残留样品的检测，在时间上不具有明显优势，更适合于高通量、多残留的检测。

Surface Plasmon Resonance Detection of Phosmet Residues in Three Kinds of Juice

In the present study, a polyclonal antibody-based method for the quantification of phosmet residues in apple,
peach and orange juice was presented using a surface plasmon resonance (SPR) sensor. Samples were directly diluted
with PBS to eliminate the matrix effect. Good accuracy and precision were obtained with mean spiked recoveries between
84.88% and 104.69% and coefficients of variation less than 6.70%, and the limit of detection (LOD) of juice samples was
0.1 μg/ L . The SPR method allowed rapid and accurate detection of phosmet and the correlation coefficient (R2) between
the data obtained using the method and high-performance liquid chromatography (HPLC) was 0.992. The chip used in the
SPR method detected phosmet in 30 min, and could be repeatedly used 30 times within 3 days, reducing the cost of tests.
The SPR method was advantageous in the terms of detection limit, accuracy and sensitivity rather other time saving in
single-residue analysis compared with the enzyme-linked immunosorbent assay (ELISA) and was more suitable for highthroughput
detection of multi-residues.