响应面试验优化广枣黄酮的微波提取工艺及黄酮的提纯

通过单因素试验和响应面分析法确定了微波辅助提取广枣黄酮的最佳条件：提取温度75 ℃、液料比62∶1、乙醇体积分数62%、微波功率500 W和提取时间7 min。在此条件下，黄酮得率远高于文献所报道的方法。通过动态法确定了AB-8型大孔树脂纯化广枣黄酮的最优工艺参数：样品液流速2 BV/h、样品液pH 2、洗脱液流速3 BV/h、洗脱液乙醇体积分数60%。同时利用制备色谱对广枣黄酮进行纯化，高效液相色谱法分析结果证明，制备色谱用于纯化广枣黄酮具有可行性。

Optimization of Microwave-Assisted Extraction by Response Surface Analysis and Purification of Flavonoids from Choerospondias axillaris Fruit

The optimal conditions for microwave-assisted extraction of flavonoids from the flesh of Choerospondias axillaris
fruits were established by using single factor experiments and response surface analysis as follows: extraction temperature,
75 ℃; ratio of solvent to material, 62:1 (mL/g); ethanol concentration, 62%; microwave power 500 W; and extraction time,
7 min. Under the proposed conditions, the yield of flavonoids was much higher than that obtained from some methods
reported in the literature. The optimal adsorption/desorption conditions for purifying the crude flavonoids with AB-8
macroporous adsorbent resin were determined as follows: the extract solution at pH 2 was passed through the column at a
flow rate of 2 bed volumes/h (BV/h), and then the column was eluted with 60% alcohol at 3 BV/h. The purification of crude
flavonoids from C. axillaris was also investigated using preparative chromatography and the feasibility was confirmed by
the results of HPLC analysis.