鼠伤寒沙门氏菌ompc基因PCR的检测方法

建立一种鼠伤寒沙门氏菌的聚合酶链式反应检测方法。根据GenBank中已发表的鼠伤寒沙门氏菌的ompc的基因序列，设计和合成了一对特异性引物，并优化了反应条件，检测了该方法的敏感性和特异性。结果成功扩增出了鼠伤寒沙门氏菌470 bp的ompc特异性基因片段，而对致病性大肠杆菌、金黄色葡萄球菌、绿脓杆菌和志贺氏菌4 种食品中常见的病原菌均未扩增出相应片段。敏感性实验结果表明本方法可检测到最低1 pg/μL的鼠伤寒沙门氏菌基因组DNA。

Establishment of PCR Detection Method for Salmonella typhimurium Based on ompc Gene

To establish a PCR detection method for Salmonella typhimurium, the ompc gene was amplified by PCR using
the specific primers designed according to the sequence published in GenBank. The PCR reaction conditions were optimized
based on the sensitivity and specificity of this method. The results showed that the 470 bp DNA fragment was specifically
amplified from Salmonella typhimurium and no DNA fragment was obtained from other common pathogenic bacteria in
foods including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Shigella. The sensitivity of this PCR
method was 1 pg/μL. In conclusion, this experiment can lay a foundation for further exploration of rapid detection methods
for Salmonella typhimurium in foods.