高效液相色谱光化学在线衍生荧光法检测鸡肉中16 种磺胺类药物残留

采用高效液相色谱-光化学柱后在线衍生-荧光检测法检测鸡肉中16 种磺胺类药物残留量。鸡肉样品经过乙腈提取并脱脂净化，色谱柱分离，光化学在线衍生并直接用荧光检测器检测。流动相为0.3%的冰乙酸和甲醇溶液梯度洗脱，流速0.7 mL/min、柱温36 ℃，激发波长320 nm、发射波长450 nm，16 种磺胺类药物得到良好分离。16 种磺胺药物在0.13～67.89 μg/mL质量度范围内，线性良好（R≥0.994 0），回收率在61.2%～106.6%之间，相对标准偏差为0.3%～13.9%，检出限为1.0～67.4 μg/kg。该方法灵敏、准确、快速，可满足鸡肉中磺胺类残留的检测。

Determination of 16 Sulfonamide Residues in Chicken by High Performance Liquid Chromatography with Online Photochemical Derivatization and Fluorescence Detection

A high performance liquid chromatography (HPLC) with online postcolumn photochemical derivatization and
fluorescence detection (FLD) method was established for the determination of 16 sulfonamide residues in chicken. Chicken
samples were extracted with acetonitrile and the extract was defatted, separated by column chromatography, treated by online
photochemical derivatization and detected using a fluorescence detector. The mobile phase was a mixture of 0.3% glacial
acetic acid and methanol solution at a flow rate of 0.7 mL/min. The column temperature was set as 36 ℃, and the excitation
and emission wavelengths were set as 320 and 450 nm, respectively. The 16 sulfonamide residues were successfully
separated and determined under chosen experimental conditions. The calibration curves showed good linearity in the range
of 0.13–67.89 μg/mL (R ≥ 0.994 0). The recovery rates of the 16 sulfonamide residues were in the range of 64.3%–106.6%
with relative standard deviation (RSD) of 0.3%–13.9%.The limits of detection (LOD) for the sulfonamides were 1–
67.4 μg/kg. The established method is sensitive, accurate, rapid, and suitable for the determination of sulfonamide residues
in chicken.