响应面试验优化碱溶酸沉法提取山药储藏蛋白工艺

以河南焦作白玉山药为原材料，在单因素试验的基础上，采用响应面法中的Box-Behnken试验设计，对影响山药储藏蛋白提取率的3 个影响因素即抽提pH值、液料比和抽提时间进行优化。结果表明：各因素对山药储藏蛋白提取率有显著影响，因素影响主次顺序为：抽提pH值＞液料比＞抽提时间，提取山药储藏蛋白的最佳工艺条件为抽提pH 8.5、液料比3∶1（mL/g）、抽提时间20 min，在此条件下山药储藏蛋白提取率的最大理论值为57.88%，实际蛋白提取率为57.74%。所得山药储藏蛋白提取回归模型显著，拟合性好，可用于预测山药储藏蛋白提取率。

Optimization of Extraction Conditions for Yam Storage Proteins through Alkali-Extraction and Acid-Precipitation by Response Surface Methodology

The extraction of storage proteins from ‘Baiyu’ yam tubers grown in Jiaozuo, Henan province was optimized
using combination of one-factor-at-a-time method, Box-Behnken design (BBD) and response surface methodology (RSM).
Three factors influencing the extraction yield of yam storage proteins, i.e., pH, liquid/material ratio and extraction time
were investigated. The results showed that all these three factors had a notable effect on the extraction yield of yam storage
proteins and their significance could be ranked in a decreasing order of pH > liquid/material ratio > extraction time. The
optimal extraction conditions were determined as 8.5, 3:1 (mL/g) and 20 min for pH, liquid/material ratio and extraction
time, respectively. The predicted extraction yield of yam storage proteins under the optimized conditions was 57.88%,
agreeing with the experimental value (57.74%). The regression model predicting the extraction of yam storage proteins was
highly significant and had excellent goodness of fit, suggesting its usefulness.