响应面试验优化单半乳糖基甘油棕榈酸酯的酶法合成工艺

以实验室自制的单半乳糖基甘油和游离棕榈酸为原料，在有机溶剂中以固定化脂肪酶Novozyme 435为催化剂，研究单半乳糖基甘油棕榈酸酯的合成条件。在单因素试验的基础上，以单半乳糖基甘油棕榈酸酯产率为响应值，确定酶添加量、底物物质的量比和反应时间作为影响合成反应的主要因素，进行响应面优化试验。获得单半乳糖基甘油棕榈酸酯的最佳合成条件为：丙酮为反应溶剂，脂肪酶Novozyme 435添加量12.93 mg/mL、底物物质的量比（单半乳糖基甘油-棕榈酸）1.00∶4.49、反应温度55 ℃、反应时间27.60 h，此时单半乳糖基甘油棕榈酸酯产率为90.17%。

Response Surface Optimization of Lipase-Catalyzed Synthesis of Palmitoyl-Monogalactosylglycerol in Organic Solvent

The enzymatic synthesis of palmitoyl-monogalactosylglycerol from monogalactosyl glycerol and free palmitic
acid in organic solvent was investigated in present study. Novozyme 435, an immobilized lipase, was used as catalyst.
The main factors that influence the yield of palmitoyl-monogalactosylglycerol, including lipase amount, and molar
ratio of monogalactosyl glycerol to palmitic acid and reaction time, were selected and optimized by the combined use
of single factor experiments and response surface methodology. The optimal synthesis conditions were determined as
follows: reaction solvent, acetone; Novozyme 435 amount, 12.93 mg/mL; molar ratio monogalactosylglycero to palmitic
acid, 1.00:4.49; reaction temperature, 55 ℃; and reaction time, 27.60 h. Under these conditions, the yield of palmitoylmonogalactosylglycerol
was 90.17%.