高效液相色谱法同时测定仙鹤草中6 种黄酮

目的：建立超声辅助萃取-高效液相色谱法分离仙鹤草中芦丁、花旗松素、香豆素、木犀草素、山奈酚和芹菜素6 种黄酮及其含量测定的方法。方法：采用Phenomenex C18色谱柱（150 mm×4.6 mm，5 μm）分离6 种成分；流动相为甲醇-0.1%醋酸溶液，梯度洗脱；流速0.8 mL/min，紫外检测波长320 nm，柱温35 ℃。结果：6 种成分在15 min内均达到基线分离，线性关系良好（r不低于0.999 1，n=6），平均回收率均在97.78%～104.4%之间（相对标准偏差小于1.20%，n=3）。结论：本实验采用超声波法提取，方法简单、快速、经济、可靠，可用于仙鹤草的质量监控。

Determination of Six Flavonoids in Agrimonia pilosa Ledeb by HPLC

Objective: To establish a high performance liquid chromatography (HPLC) method for separation and
quantification of rutin, taxifolin, coumarin, luteolin, kaempferol and apigenin in Agrimonia pilosa Ledeb by ultrasonic-assisted
extraction. Methods: The separation of six flavonoids was performed on Phenomenex C18 column (150 mm × 4.6 mm,
5 μm) using step gradient elution. The mobile phase was composed of methanol and 0.1% acetic acid at a flow rate of 0.8 mL/min.
The UV detection wavelength was 320 nm and the column temperature was set at 35 ℃. Results: Good separation of
rutin, taxifolin, coumarin, luteolin, apigenin and kaempferol was achieved within 15 min. Calibration curves of all the six
components showed an excellent linear relationship (r ≥ 0.999 1, n = 6). The average recoveries were between 97.78%
and 104.4% with a relative standard deviation (RSD) of < 1.20%. Conclusion: This method, based on ultrasonic-assisted
extraction, is simple, fast, economical and reliable.