人乳头瘤病毒16型L1 N-端主要抗原基因的原核表达

目的　获得序列正确的人乳头瘤病毒 (HPV) 16型L1N -端主要抗原基因 ,构建其原核表达载体 ,并进行诱导表达。方法　采用PCR法从宫颈癌组织中获得HPV16型L1N 端主要基因重组表达质粒 ,转化E .coliDH5α ,4 2℃诱导表达 ,Westernblot分析表达产物。结果　获得了序列正确的人乳头瘤病毒HPV16型L1N -端主要基因 ,相对分子质量约为 5 10 0 0。表达蛋白能与乳头瘤病毒抗体及乳头瘤病毒感染患者的血清发生抗原抗体反应。结论　已成功构建HPV16型L1N -端主要基因表达载体 ,并获得有效表达。

Prokaryotic Expression of N-terminal Cardinal Antigen Gene of L1 Protein of Human Papillomavirus Type 16

Objective To obtain the N-terminal cardinal antigen gene of L1 protein of human papillomavirus type 16 (HPV16L1) and express in a prokaryotic vector.Methods The N-terminal cardinal antigen gene of HPV16L1 was amplified from cervical carcinoma tissue by PCR,identified by sequencing and cloned into plasmid pBV220.The constructed recombinant plasmid was transformed into E.coli DH5α and expressed at 42℃.The expressed product was identified by Western blot.Results The N-terminal cardinal antigen gene of HPV16L1 with a correct sequence was obtained and expressed in prokaryotic vector.The expressed protein,with a relative molecular of 51 000,reacted with HPV antibody and the sera of patients infected with HPV.Conclusion A prokaryotic expression vector of N-terminal cardinal antigen gene of HPV16L1 was successfully constructed,and the antigen was effectively expressed.