Screening various Yarrowia lipolytica strains for citric acid production |
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| Authors: | Erdem Carsanba Seraphim Papanikolaou Patrick Fickers Huseyin Erten |
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| Affiliation: | 1. Faculty of Agriculture, Food Engineering Department, Cukurova University, Adana, Turkey Altinozu Agricultural Sciences Vocational School, Mustafa Kemal University, Antakya, Turkey;2. Department of Food Science& Human Nutrition, Agricultural University of Athens, Athens, Greece;3. Gembloux Agro-Bio Tech Microbial Processes and Interactions (MiPI), University of Liège, Gembloux, Belgium;4. Faculty of Agriculture, Food Engineering Department, Cukurova University, Adana, Turkey |
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| Abstract: | Citric acid (CA) productivity by Yarrowia lipolytica dependents on strain type, carbon source, carbon to nitrogen (C/N) molar ratio as well as physicochemical conditions (pH, temperature, oxygen transfer rate, etc.). In the current study, 10 different Y. lipolytica strains were first challenged in shake-flask culture for CA production in a glucose-based media under nitrogen-limited conditions. For the most promising one, strain K57, CA productivity was monitored during culture in batch bioreactor for three initial C/N molar ratio (167, 367, and 567 Cmol/Nmol). The highest CA yield (0.77 g/g glucose), titre (72.3 g/L CA), and productivity (0.04 g/g.h) were found for C/N ratio of 367. However, the highest growth rate was obtained for an initial C/N ratio of 167. From these results, Y. lipolytica strain K57 could be considered to produce CA at higher titre on glucose-based medium in batch bioreactor than others Y. lipolytica strain reported in the literature. |
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| Keywords: | batch bioreactor culture C/N molar ratio citric acid Yarrowia lipolytica |
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