| Abstract: | The accuracy and precision of results obtained from light-scattering detection at two angles (TALLS) for size-exclusion chromatography (SEC) are examined for linear narrow molecular weight distribution polystyrenes between 1,290,000 and 20,000,000 MW and for branched polyesters. The ratio of light-scattering intensities at 15° and 90° is used to calculate weight-average molecular weight, M?w, and an average root-mean-square radius, r?gu, equivalent to the z-average radius. A shape for the polymer molecule is assumed and an analytical relationship for the particle-scattering function is required. It is shown that analysis of the data using the particle-scattering function for a random coil is valid for both high molecular weight, linear polystyrenes and long-chain branched polyesters. The radius, r?gu, is determined with high precision by using the ratio of light-scattering signals, which is insensitive to errors in sample concentration and changes in the eluent flow rate. The correct average radius for the whole polymer is obtained despite using low-efficiency, large-particle diameter SEC columns; however, axial dispersion significantly affects molecular weights and radii calculated at each retention volume that can limit the utility of plots used to deduce polymer conformation. © 1995 John Wiley & Sons, Inc. |
