| 一株低温碱性脂肪酶产生菌TS182的分离与鉴定研究 |
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| 引用本文： | 刘姝,房耀维,王淑军,吕明生,陈丽,焦豫良. 一株低温碱性脂肪酶产生菌TS182的分离与鉴定研究[J]. 淮海工学院学报, 2009, 18(1): 83-86 |
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| 作者姓名： | 刘姝 房耀维 王淑军 吕明生 陈丽 焦豫良 |
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| 作者单位： | 淮海工学院,海洋学院,江苏,连云港,222005 |
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| 基金项目： | 江苏省高校自然科学基础研究项目，连云港市科技公关项目，淮海工学院博士启动基金 |
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| 摘 要： | 通过Tween-80平板初筛和摇瓶发酵复筛，从连云港海域海水及海泥样品中分离的菌株中筛选获得一株低温碱性脂肪酶高产菌株TS182。通过形态学、生理生化以及分子生物学鉴定菌株TS182为荧光假单孢菌（Pseudomonas fluorescens）。菌株TS182所产脂肪酶最适温度和最适pH分别为15℃和9．0。
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| 关 键 词： | 低温碱性脂肪酶 荧光假单孢菌 筛选 鉴定 |
Screening and Identification of a Strain TS182 Producing a Cold-adaptive Alkaline Lipase |
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| LIU Shu,FANG Yao-wei,WANG Shu-jun,L Ming-sheng,CHEN Li,JIAO Yu-liang. Screening and Identification of a Strain TS182 Producing a Cold-adaptive Alkaline Lipase[J]. Journal of Huaihai Institute of Technology:Natural Sciences Edition, 2009, 18(1): 83-86 |
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| Authors: | LIU Shu FANG Yao-wei WANG Shu-jun L Ming-sheng CHEN Li JIAO Yu-liang |
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| Affiliation: | School of Marine Science and Technology;Huaihai Institute of Technology;Lianyungang 222005;China |
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| Abstract: | Strain TS182, which produces alkaline lipase, was isolated from strains in the marine water and marine mud collected from Lianyungang. It was identified as Pseudomonas fluorescens by morphological, physiological, biochemical tests and 16S rDNA gene sequence analysis. The optimal temperature and pH of the lipase of strain TS182 were 25 ℃ and 9.0 respectively. |
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| Keywords: | cold-adaptive alkaline lipase Pseudomonas fluorescens screening identification |
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