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Gel Strength Enhancement by Addition of Microbial Transglutaminase during Onshore Surimi Manufacture
Authors:HIROKO SAKAMOTO  YOSHIYUKI KUMAZAWA  SEIICHIRO TOIGUCHI  KATSUYA SEGURO  TAKAHIKO SOEDA  MASAO MOTOKI
Affiliation:The authors are affiliated with the Food Research &Development Laboratories, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, 210 Japan.
Abstract:Surimi from Alaska pollock flesh was manufactured onshore with Microbial transglutaminase (MTGase). Effect of MTGase was investigated by evaluating breaking strength and deformation of gels from MTGase-treated surimi with and without setting at 30°C. Quantitative analysis of ε-(γ-glutamyl)lysine (GL) crosslink was also carried out to monitor the MTGase reaction. In set gels, breaking strength and GL crosslink increased, and myosin heavy chain decreased correspondingly with MTGase concentration. These changes were smaller in gels prepared without setting. Results suggest that surimi gel could be improved through the formation of GL crosslinks by added MTGase in surimi.
Keywords:surimi  gel strength  transglutaminase  glutamyl-lysine
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