| 应用疏水层析纯化HIV-1跨膜蛋白gp41截短体包涵体 |
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| 引用本文： | 端义坤,汪超英,孙可芳,张涛.应用疏水层析纯化HIV-1跨膜蛋白gp41截短体包涵体[J].粉末涂料与涂装,2004,17(3):166-167. |
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| 作者姓名： | 端义坤 汪超英 孙可芳 张涛 |
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| 作者单位： | 武汉生物制品研究所免疫研究室 (武汉430060)
(端义坤，汪超英，孙可芳)，武汉生物制品研究所免疫研究室 (武汉430060)(张涛) |
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| 摘 要： | 目的对表达HIV-1跨膜蛋白gp41截短体包涵体进行纯化。方法将收获的表达菌体洗涤、超声波破菌、预处理后进行疏水层析。结果纯化后的gp41截短体的融合蛋白纯度达到90％,并且具有较好的抗原性和特异性。结论利用疏水层析可以将目的蛋白进行有效的纯化,为下一步的应用打下基础。
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| 关 键 词： | 人类免疫缺陷病毒1型 跨膜蛋白 包涵体 疏水层析 纯化 |
| 修稿时间： | 2003年8月25日 |
Purification of Inclusion Body of HIV-1 Transmembrane Protein gp41 Truncate by Hydrophobic Chromatography |
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| DUAN Yi-kun,WANG Chao-ying,SUN Ke-fang,et al.Purification of Inclusion Body of HIV-1 Transmembrane Protein gp41 Truncate by Hydrophobic Chromatography[J].Chinese Journal of Biologicals,2004,17(3):166-167. |
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| Authors: | DUAN Yi-kun WANG Chao-ying SUN Ke-fang |
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| Abstract: | Objective To purify the inclusion body of expressed HIV-1 transmembrane protein gp41 truncate. Methods Ultrasonicate the expressed bacteria and purify the inclusion body by hydrophobic chrom-atography. Results The fusion protein of gp41 truncate obtained by the purification of inclusion body reached a purity of 90% and showed good antigenicity and specificity. Conclusion The HTV-1 transmembrane protein gp41 truncate protein was effectively purified by hydrophobic chromatography. |
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| Keywords: | HIV Transmembrane Inclusion body Hydrophobic chromatography Purification |
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