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Light microscopy and scanning electron microscopy: Implications for authentication of misidentified herbal drugs
Authors:Muhammad Zafar  Mushtaq Ahmad  Shazia Sultana  Lubna  Farida Anjum  Fethi Ahmet Ozdemir  Akash Tariq  Abdul Nazir  Ghulam Yaseen  Sheikh Zain Ul Aabidin  Hina Gul  Shafiq ur Rehman
Abstract:Plant‐based drugs have reached remarkable acceptability as therapeutic remedy for various diseases due to the adverse effects of contemporary medicines. This increasing popularity of herbal drugs leads to a growing herbal market for the development of plant‐based drugs, nutraceuticals, pharmaceuticals and cosmeceuticals. Herbal drug adulteration is a complex problem which currently has undeniable consequences on health and nutrition. Ambiguities in nomenclature, misidentification and resemblance of colour and texture of the crude herbal drugs are the major causes of adulteration. Three different species commercially marketed under the same trade name Halion are Lepidium apetalum, Asparagus officinalis, and Lepidium didymum. The genuine source of Halion is Lepidium apetalum, which is authenticated by using basic and advanced taxonomic techniques. Morphology, anatomy and palynology of the misidentified sources were done using light and scanning electron microscopic techniques for authentication. This study may help to set microscopic techniques as a tool to achieve quality and standardization of the genuine source of the herbal drug. Phytochemical analysis and biological screening is needed for the further establishment of authenticity and quality of herbal drugs.
Keywords:adulteration  herbal drug  light microscopy  quality control  scanning electron microscopy
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