The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology |
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| Authors: | F. -U. Gast P. S. Dittrich P. Schwille M. Weigel M. Mertig J. Opitz U. Queitsch S. Diez B. Lincoln F. Wottawah S. Schinkinger J. Guck J. Käs J. Smolinski K. Salchert C. Werner C. Duschl M. S. Jäger K. Uhlig P. Geggier S. Howitz |
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| Affiliation: | (1) GeSiM mbH, 01454 Grosserkmannsdorf, Germany;(2) Department Miniaturization, Institute for Analytical Sciences, 44139 Dortmund, Germany;(3) Institute for Biophysics/BioTec, TU Dresden, 01307 Dresden, Germany;(4) Max Bergmann Center of Biomaterials and Institute of Materials Science, TU Dresden, 01069 Dresden, Germany;(5) Group Optical Technology Development and Bionanotechnology, Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany;(6) Institute for Soft Matter Physics, Department of Physics and Geosciences, University of Leipzig, 04103 Leipzig, Germany;(7) Department of Biocompatible Materials, Leibniz Institute of Polymer Research and Max Bergmann Center of Biomaterials Dresden, 01069 Dresden, Germany;(8) Fraunhofer Institute for Biomedical Engineering (Fh-IBMT), 10115 Berlin, Germany |
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| Abstract: | We describe a novel microfluidic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of the coverslip surface with reagents, biomolecules, or cells. A poly(dimethylsiloxane) (PDMS) layer is cast in a special molding station, using masters made by photolithography and dry etching of silicon or by photoresist patterning on glass or silicon. This channel system can be reused while the coverslip is exchanged between experiments. As normal fluidic connectors are used, the link to external, computer-programmable syringe pumps is standardized and various fluidic channel networks can be used in the same setup. The system can house hydrogel microvalves and microelectrodes close to the imaging area to control the influx of reaction partners. We present a range of applications, including single-molecule analysis by fluorescence correlation spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted in the PDMS layer, and the integration of microelectrodes for actuation and sensing. |
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| Keywords: | Lab-on-chip PDMS microchannel Microscopy Hydrogel valve Microelectrodes |
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