九州虫草菌丝体多糖Ck_3-A的分离纯化及其理化性质研究

以九州虫草 (Cordycepskyushuensis Kobayasi)发酵菌丝体为试验材料 ,采用水提法得到九州虫草菌丝体粗多糖。粗多糖经酶法结合Sevag法除蛋白、H2 O2 法脱色、透析、分级沉淀得到Ck3 ,Ck3 经DE 2 3柱层析得到多糖Ck3 A。经紫外扫描、聚丙烯酰胺凝胶电泳、Sepharose 4B柱层析等方法检测确定Ck3 A为均一组分。用苯酚 硫酸法测得Ck3 A的含糖量为 73 1% ,硫酸 咔唑法测得Ck3 A含有微量的葡萄糖醛酸。Sepharose 4B柱层析测得Ck3 A的分子量为 92 5 0 0u。红外光谱结果显示Ck3 A含有α 型糖苷键。

Isolation, Purification and Properties of A Polysaccharide Ck3-A from Cordyceps kyushuensis Cultured Mycelium

Crude polysaccharide were extracted from the cultured mycelium of Cordyceps kyushuensis with water Ck 3 was obtained from crude polysaccharide after removing proteins(Sevag method )and color (H 2O 2 method ),dialysis and precipitation fractionation with alcohol A polysaccharide Ck 3 A was isolated from Ck 3 by DEAE 23 chromatography Its homogeneity was verified by UV scan,polyacrylamide gel electrophoresis and gel-filtration chromatography Its carbohydrate content was 73 1%, and had little uronic acid The average molecular weight of Ck 3 A was 92 500 Da The results of IR showed that Ck 3 A had a α glycoside linkage