Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples |
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Authors: | Marc Van den Bulcke Adinda De Schrijver Daniele De Bernardi Yann Devos Guillaume MbongoMbella Amaya Leunda Casi William Moens Myriam Sneyers |
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Affiliation: | (1) Division of Biosafety and Biotechnology, Institute of Public Health, J. Wytsmanstraat 12-14, 1050 Brussels, Belgium |
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Abstract: | The determination of the presence of genetically modified plant material by the detection of expressed genetically engineered proteins using lateral flow protein strip tests has been evaluated in different matrices. The presence of five major genetically engineered proteins (CP4-EPSPS, CryIAb, Cry9C, PAT/pat and PAT/bar protein) was detected at low levels in seeds, seed/leaf powder and leaf tissue from genetically modified soy, maize or oilseed rape. A comparison between “protein strip test” (PST) and “polymerase chain reaction” (PCR) analysis of genetically modified food/feed samples demonstrates complementarities of both techniques. |
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Keywords: | GMO Lateral flow Recombinant proteins Detection Food/feed |
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