| 仿生膜吸附-絮凝组合固定化脂肪酶及其应用 |
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| 引用本文： | 袁俊红,沈宏宇,黄任远,胡永红,沈树宝.仿生膜吸附-絮凝组合固定化脂肪酶及其应用[J].化工进展,2004,23(6):645-649. |
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| 作者姓名： | 袁俊红 沈宏宇 黄任远 胡永红 沈树宝 |
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| 作者单位： | 南京工业大学制药与生命科学院,南京,210009;南京工业大学制药与生命科学院,南京,210009;南京工业大学制药与生命科学院,南京,210009;南京工业大学制药与生命科学院,南京,210009;南京工业大学制药与生命科学院,南京,210009 |
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| 基金项目： | 国家自然科学基金 (No .2 99760 19,No .2 0 1760 18,No .2 0 3 760 3 4)，江苏省自然科学基金 (No .BK2 0 0 3 0 84,No .BS2 0 0 3 0 3 0 )资助项目 |
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| 摘 要： | 研究了猪胰脂肪酶的仿生膜、吸附-絮凝组合固定化新工艺，考察了该组合固定化脂肪酶体系用于橄榄油水解的性能。最终试验结果表明：该组合固定化脂肪酶水解反应体系的反应转化率达到54.5％，固定化酶质量回收率86，7％，酶活回收率66．7％，远高于单一固定化方法。
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| 关 键 词： | 酶固定化 吸附-絮凝 脂肪酶 仿生膜 |
| 文章编号： | 1000-6613(2004)06-0645-05 |
| 修稿时间： | 2004年2月4日 |
Immobilization of PPL by the Combination of Bio-mimic Membrane and Absorption-Flocculation and its Application |
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| Yuan Junhong,Shen Hongyu,Huang Renyuan,Hu Yonghong,Shen Shubao.Immobilization of PPL by the Combination of Bio-mimic Membrane and Absorption-Flocculation and its Application[J].Chemical Industry and Engineering Progress,2004,23(6):645-649. |
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| Authors: | Yuan Junhong Shen Hongyu Huang Renyuan Hu Yonghong Shen Shubao |
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| Abstract: | A new method of immobilizing PPL by bio-mimic membrane with absorption-flocculation was studied. Moreover, the elementary characters of this immobilization system were demonstrated and analyzed. The experiments showed that the reaction conversion yield of hydrolyzing olive oil by this immobilized PPL enzyme is 54 5%, enzyme-quality recovery is 86 7%, and enzyme-activity recovery is 66 7%, which are all superior to the immobilized enzyme with single absorption or flocculation method. |
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| Keywords: | enzyme immobilization absorption-flocculation PPL bio-mimic membrane |
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